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Molecular phylogeny of advanced snakes (Serpentes, Caenophidia) with an emphasis on South American Xenodontines: a revised classification and descriptions of new taxa Pap. Avulsos de Zool. (São Paulo)
Zaher,Hussam; Grazziotin,Felipe Gobbi; Cadle,John E.; Murphy,Robert W.; Moura-Leite,Julio Cesar de; Bonatto,Sandro L..
We present a molecular phylogenetic analysis of caenophidian (advanced) snakes using sequences from two mitochondrial genes (12S and 16S rRNA) and one nuclear (c-mos) gene (1681 total base pairs), and with 131 terminal taxa sampled from throughout all major caenophidian lineages but focussing on Neotropical xenodontines. Direct optimization parsimony analysis resulted in a well-resolved phylogenetic tree, which corroborates some clades identified in previous analyses and suggests new hypotheses for the composition and relationships of others. The major salient points of our analysis are: (1) placement of Acrochordus, Xenodermatids, and Pareatids as successive outgroups to all remaining caenophidians (including viperids, elapids, atractaspidids, and all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serpentes; Colubridae; Caenophidia; Phylogeny; Classification; Systematics; Xenodontinae; Dipsadinae; New genus; Elapoidea; Colubroidea; South America; West Indies.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492009001100001
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Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level BJM
Delamare,Ana Paula Longaray; Artico,Liane de Oliveira; Grazziotin,Felipe Gobbi; Echeverrigaray,Sergio; Costa,Sérgio Olavo Pinto da.
Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Molecular markers; Species identification.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016
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